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canine chondrocyte differentiation medium  (Cell Applications Inc)
93
Cell Applications Inc canine chondrocyte differentiation medium
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after <t>chondrocyte</t> induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Canine Chondrocyte Differentiation Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/canine chondrocyte differentiation medium/product/Cell Applications Inc
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canine chondrocyte differentiation medium - by Bioz Stars, 2026-02
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chondrocyte differentiation medium bullet kit medium  (Lonza)
90
Lonza chondrocyte differentiation medium bullet kit medium
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after <t>chondrocyte</t> induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Chondrocyte Differentiation Medium Bullet Kit Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondrocyte differentiation medium bullet kit medium/product/Lonza
Average 90 stars, based on 1 article reviews
chondrocyte differentiation medium bullet kit medium - by Bioz Stars, 2026-02
90/100 stars
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chondrocyte differentiation-inducing medium cdim  (Funakoshi ltd)
90
Funakoshi ltd chondrocyte differentiation-inducing medium cdim
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after <t>chondrocyte</t> induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Chondrocyte Differentiation Inducing Medium Cdim, supplied by Funakoshi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondrocyte differentiation-inducing medium cdim/product/Funakoshi ltd
Average 90 stars, based on 1 article reviews
chondrocyte differentiation-inducing medium cdim - by Bioz Stars, 2026-02
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chondrocyte differentiation medium  (Lonza)
90
Lonza chondrocyte differentiation medium
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after <t>chondrocyte</t> induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Chondrocyte Differentiation Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondrocyte differentiation medium/product/Lonza
Average 90 stars, based on 1 article reviews
chondrocyte differentiation medium - by Bioz Stars, 2026-02
90/100 stars
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chondrocyte differentiation medium cdmtm  (Lonza)
90
Lonza chondrocyte differentiation medium cdmtm
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after <t>chondrocyte</t> induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Chondrocyte Differentiation Medium Cdmtm, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondrocyte differentiation medium cdmtm/product/Lonza
Average 90 stars, based on 1 article reviews
chondrocyte differentiation medium cdmtm - by Bioz Stars, 2026-02
90/100 stars
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chondrocyte differentiation medium  (Thermo Fisher)
90
Thermo Fisher chondrocyte differentiation medium
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after <t>chondrocyte</t> induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Chondrocyte Differentiation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondrocyte differentiation medium/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
chondrocyte differentiation medium - by Bioz Stars, 2026-02
90/100 stars
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Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).

Journal: Regenerative Therapy

Article Title: Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins

doi: 10.1016/j.reth.2025.05.008

Figure Lengend Snippet: Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).

Article Snippet: The cells were then cultured in canine chondrocyte differentiation medium (Cell Applications) for 30 days at 37 °C and 5 % CO 2 .

Techniques: Derivative Assay, Staining, Quantitative Proteomics, Standard Deviation, Marker